Problem Statement
Evidence exists to show that the response of a single isolated cardiac cell to a mechanical stimulus provides a means of studying the mechanisms of heart failure and other health problems. Existing technology lacks certain specifications necessary for studying isolated cardiac cells under physiological conditions. Current types of magnetic and optical tweezer only allow for generation of forces on the order of pico- and micro-Newtons[1]. To stimulate physical cardiac cell-cell or cell-matrix interactions one must generate a force on the order of 100 nano-Newtons. The aim of this design project is to devise a means of applying a localized force to a cardiac myocyte comparable in magnitude to that found in vivo. The force application must be controllable in speed, magnitude, and direction. Moreover, the device must be user-friendly, safe, and be compatible with the existing optical setup used currently by our investigators because the means of measuring the readouts to study cardiac mechanotransduction already exist. Also, the design must provide accurate and reliable results while being durable and sustainable.
References
[1] Neuman, Keir C Neuman C, and Attila Nagy. "Single-molecule force spectroscopy: optical tweezer, magnetic tweezer and atomic force microscopy." Nature Methods (May-June 2008). 2 Oct. 2008