Lab-on-Chip Large Scale Parallel Cantilever-Based Allergy Testing Device

Introduction

The diagnosis of allergies is based on the quantification of allergen-specific IgE binding that triggers a hypersensitive immune response. Current diagnostics, primarily tests directly observing the skin’s reaction to allergens and based on radioimmunoassays of the blood (RAST), are hampered by false positives, impairing their accuracy, and are uncomfortable and time-consuming for both patients and the technicians administering the test [1].

In recent years, the field of microelectromechanical devices (MEMs) has brought micro- and nanoscale biosensors to the forefront. This technology allows for the fabrication of large-scale parallel devices that integrate an array of biochemical tests onto a single chip and are accessible to developing countries [2]. The objective of this project was to develop a high-throughput allergen sensor that is cost effective and more convenient and accurate than conventional allergy diagnostics.

Example of nanocantilever-based sensor

CantiChip8 [a]. A nanocantilever-based device on the market with integrated piezoresistors and sgnal readout for detecting molecular interactions.

References
  1. Winter, W. E., N. S. Hardt, et al. (2000). "Immunoglobulin E: importance in parasitic infections and hypersensitivity responses." Arch Pathol Lab Med 124(9): 1382-1385.
  2. Yager, P., T. Edwards, et al. (2006). "Microfluidic diagnostic technologies for global public health." Nature 442(7101): 412-418.